A301-434A

antibody from Invitrogen Antibodies

Targeting: IRS2

Western blot (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A301-434A

Provider

Invitrogen Antibodies

Product name

IRS2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human IRS2 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-IRS2 (Cat. No. A301-434A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human IRS2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-IRS2 antibody A301-434A used for IP at 3 µg/mg lysate. IRS2 was also immunoprecipitated by rabbit anti-IRS2 antibodies A301-432A and A301-433A, which recognize upstream epitopes. For blotting immunoprecipitated IRS2, A300-432A (A) and A300-433A (B) were used at 1 µg/ml. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).