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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX105083 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX105083, RRID:AB_1951843
- Product name
- SEMA6A antibody [N2C1], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Sample (50 ?g of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX105083 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- GeneTex (provider)
- Main image
- Experimental details
- SEMA6A antibody [N2C1], Internal detects SEMA6A protein by western blot analysis.A. 30 ?g HepG2 whole cell lysate/extract B. 30 ?g Molt-4 whole cell lysate/extract C. 30 ?g Raji whole lysate/extract 7.5% SDS-PAGESEMA6A antibody [N2C1], Internal (GTX105083) dilution: 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SEMA6A antibody [N2C1], Internal detects SEMA6A protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SEMA6A protein stained by SEMA6A antibody [N2C1], Internal (GTX105083) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using SEMA6A(GTX105083) antibody at 1:100 dilution.
