PA5-28187

antibody from Invitrogen Antibodies

Targeting: SEMA6A HT018, KIAA1368, SEMA, SEMA6A1, SEMAQ

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

PA5-28187

Provider

Invitrogen Antibodies

Product name

Anti-SEMA6A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A431, HepG2, mouse brain. Predicted reactivity: Mouse (95%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SEMA6A using 50 µg of mouse brain lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a SEMA6A polyclonal antibody (Product # PA5-28187) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SEMA6A using A) 30 µg HepG2 whole cell lysate (B) 30 µg Molt-4 whole cell lysate and C) 30 µg Raji whole lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a SEMA6A polyclonal antibody (Product # PA5-28187) at a dilution of 1:1000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SEMA6A showing staining in the cytoplasm of A431 cells. A431 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a SEMA6A polyclonal antibody (Product # PA5-28187) diluted at 1:500. Blue: Hoechst 33342 staining.