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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AF3155 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal ROBO3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse ROBO3 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) ROBO3 and less than 5% cross-reactivity with rhROBO2 and rhROBO4 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references A-kinase Anchoring Protein 79/150 Recruits Protein Kinase C to Phosphorylate Roundabout Receptors.
Dbx1 triggers crucial molecular programs required for midline crossing by midbrain commissural axons.
Slit2 and Robo3 modulate the migration of GnRH-secreting neurons.
Neural RNA-binding protein Musashi1 controls midline crossing of precerebellar neurons through posttranscriptional regulation of Robo3/Rig-1 expression.
Samelson BK, Gore BB, Whiting JL, Nygren PJ, Purkey AM, Colledge M, Langeberg LK, Dell'Acqua ML, Zweifel LS, Scott JD
The Journal of biological chemistry 2015 May 29;290(22):14107-19
The Journal of biological chemistry 2015 May 29;290(22):14107-19
Dbx1 triggers crucial molecular programs required for midline crossing by midbrain commissural axons.
Inamata Y, Shirasaki R
Development (Cambridge, England) 2014 Mar;141(6):1260-71
Development (Cambridge, England) 2014 Mar;141(6):1260-71
Slit2 and Robo3 modulate the migration of GnRH-secreting neurons.
Cariboni A, Andrews WD, Memi F, Ypsilanti AR, Zelina P, Chedotal A, Parnavelas JG
Development (Cambridge, England) 2012 Sep;139(18):3326-31
Development (Cambridge, England) 2012 Sep;139(18):3326-31
Neural RNA-binding protein Musashi1 controls midline crossing of precerebellar neurons through posttranscriptional regulation of Robo3/Rig-1 expression.
Kuwako K, Kakumoto K, Imai T, Igarashi M, Hamakubo T, Sakakibara S, Tessier-Lavigne M, Okano HJ, Okano H
Neuron 2010 Aug 12;67(3):407-21
Neuron 2010 Aug 12;67(3):407-21
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse ROBO3 by Western Blot. Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse ROBO3 Antigen Affinity-purified Polyclonal Antibody (Catalog # Af3155) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ROBO3 at approximately 205 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ROBO3 in Mouse Embryo. ROBO3 was detected in immersion fixed frozen sections of mouse embryo (E11.5) using Mouse ROBO3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3155) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to developing forebrain. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
