H00064221-M02

antibody from Abnova Corporation

Targeting: ROBO3 FLJ21044, HGPPS, HGPS, RBIG1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00064221-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00064221-M02, RRID:AB_1713130

Product name

ROBO3 monoclonal antibody (M02), clone 2H1

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant ROBO3.

Antigen sequence

MTPPLQGPRARFRKKPKALPYRRENSPGDLPPPPL
PPPEEEASWALELRAAGSMSSLERERSGERKAVQA
VPLAAQRVLHPDEEAWLPYSRPSFLSRGQGTSTCS
TAGSNSSRGSSSSRGSRGPGRSRSQSRSQSQRPGQ
KRREEPR


Isotype

IgG

Antibody clone number

2H1

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of ROBO3 expression in transfected 293T cell line by ROBO3 monoclonal antibody (M02), clone 2H1.Lane 1: ROBO3 transfected lysate (Predicted MW: 16.1 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged ROBO3 is 0.1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol