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antibody from Invitrogen Antibodies
Targeting: DNM2
CMT2M, CMTDI1, CMTDIB, DI-CMTB, DYN2, DYNII
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [2]
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- Product number
- PA5-19800 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Dynamin 2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Heat mediated antigen retrieval recommended prior to tissue staining.
- Concentration
- 0.8 mg/mL
Submitted references PICK1 regulates AMPA receptor endocytosis via direct interactions with AP2 α-appendage and dynamin.
Diaphragm assessment in mice overexpressing phospholamban in slow-twitch type I muscle fibers.
Phospholamban overexpression in mice causes a centronuclear myopathy-like phenotype.
Interleukin-1-induced activation of the small GTPase Rac1 depends on receptor internalization and regulates gene expression.
Fiuza M, Rostosky CM, Parkinson GT, Bygrave AM, Halemani N, Baptista M, Milosevic I, Hanley JG
The Journal of cell biology 2017 Oct 2;216(10):3323-3338
The Journal of cell biology 2017 Oct 2;216(10):3323-3338
Diaphragm assessment in mice overexpressing phospholamban in slow-twitch type I muscle fibers.
Fajardo VA, Smith IC, Bombardier E, Chambers PJ, Quadrilatero J, Tupling AR
Brain and behavior 2016 Jun;6(6):e00470
Brain and behavior 2016 Jun;6(6):e00470
Phospholamban overexpression in mice causes a centronuclear myopathy-like phenotype.
Fajardo VA, Bombardier E, McMillan E, Tran K, Wadsworth BJ, Gamu D, Hopf A, Vigna C, Smith IC, Bellissimo C, Michel RN, Tarnopolsky MA, Quadrilatero J, Tupling AR
Disease models & mechanisms 2015 Aug 1;8(8):999-1009
Disease models & mechanisms 2015 Aug 1;8(8):999-1009
Interleukin-1-induced activation of the small GTPase Rac1 depends on receptor internalization and regulates gene expression.
Windheim M, Hansen B
Cellular signalling 2014 Jan;26(1):49-55
Cellular signalling 2014 Jan;26(1):49-55
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse Testis Tissue Lysate using Product # PA5-19800, Dynamin 2 primary antibody at a dilution of 1 µg/mL (lane 1). Staining of SK N BE Whole Cell Lysate at a dilution of 1 µg/mL (Lane 2). Staining of Y79 Whole Cell Lysate at a dilution of 1 µg/mL (lane 3). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Dynamin 2 Polyclonal Antibody (Product # PA5-19800) and a 110kDa band corresponding to Dynamin 2 was observed across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), SH-SY5Y (Lane 2), HEK-293 (Lane 3), MCF7 (Lane 4), K-562 (Lane 5), Hep G2 (Lane 6), Raji (Lane 7), Mouse Lung (Lane 8) and Rat Brain (Lane 9) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1ug/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using Product # PA5-19800, anti-Dynamin 2 antibody. The cells were fixed with PFA (4%) for 10 minutes, permabilised with BSA (1%), normal goat serum (10%) and glycine (0.3 M) in 0.1% T-BST for 1 hour and exposed to the primary antibody at a concentration of 1 µg/mL overnight at 4C. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4. PICK1 binds dynamin via BAR and GTPase domains and regulates dynamin polymerization. (A) Endogenous PICK1 interacts with endogenous dynamin 1, 2, and 3 in neurons. Extracts of cortical neurons were immunoprecipitated with PICK1 antibody or control IgG. Proteins were detected by Western blotting. Input is 5% of offered protein. Blots shown are representative of more than five independent experiments. (B) PICK1 interacts directly with dynamin. GST or GST-PICK1 was immobilized on glutathione agarose and incubated with purified HA-dynamin. Proteins were detected by Western blotting. Blots shown are representative of four independent experiments. (C) PICK1 interacts with the GTPase domain of dynamin. HEK293 cells were cotransfected with plasmids expressing Flag-PICK1 and GFP, GFP-dynamin 2, or dynamin domains as indicated. Cells were lysed and incubated with GFP-trap agarose. Blots shown are representative of five independent experiments. (D) Dynamin interacts with the PICK1 BAR domain. HEK cells were cotransfected with plasmids expressing HA-dynamin and GFP, GFP-PICK1, or truncations, as indicated in the top panel. Cells were lysed and incubated with GFP-trap agarose. Blots shown are representative of five independent experiments. (E) PICK1 promotes dynamin polymerization. His 6 -PICK1 at the concentrations indicated was incubated with HA-dynamin. Polymerized dynamin was pelleted by centrifugation, and protein in the supernatant and pellet was analyzed by Western bl
