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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [8]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- NB100-56557 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56557, RRID:AB_838963
- Product name
- Mouse Monoclonal TEM7/PLXDC1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references The gain-of-function GLI1 transcription factor TGLI1 enhances expression of VEGF-C and TEM7 to promote glioblastoma angiogenesis.
Tumor endothelial markers define novel subsets of cancer-specific circulating endothelial cells associated with antitumor efficacy.
TEM7 (PLXDC1) in neovascular endothelial cells of fibrovascular membranes from patients with proliferative diabetic retinopathy.
High expression of tumor endothelial marker 7 is associated with metastasis and poor survival of patients with osteogenic sarcoma.
Chemotherapeutic stress selectively activates NF-kappa B-dependent AKT and VEGF expression in liver cancer-derived endothelial cells.
Expression of tumor endothelial marker 7 mRNA and protein in the dorsal root ganglion neurons of the rat.
Cloning, characterization and neuronal expression profiles of tumor endothelial marker 7 in the rat brain.
Identification of a binding partner for the endothelial cell surface proteins TEM7 and TEM7R.
Carpenter RL, Paw I, Zhu H, Sirkisoon S, Xing F, Watabe K, Debinski W, Lo HW
Oncotarget 2015 Sep 8;6(26):22653-65
Oncotarget 2015 Sep 8;6(26):22653-65
Tumor endothelial markers define novel subsets of cancer-specific circulating endothelial cells associated with antitumor efficacy.
Mehran R, Nilsson M, Khajavi M, Du Z, Cascone T, Wu HK, Cortes A, Xu L, Zurita A, Schier R, Riedel B, El-Zein R, Heymach JV
Cancer research 2014 May 15;74(10):2731-41
Cancer research 2014 May 15;74(10):2731-41
TEM7 (PLXDC1) in neovascular endothelial cells of fibrovascular membranes from patients with proliferative diabetic retinopathy.
Yamaji Y, Yoshida S, Ishikawa K, Sengoku A, Sato K, Yoshida A, Kuwahara R, Ohuchida K, Oki E, Enaida H, Fujisawa K, Kono T, Ishibashi T
Investigative ophthalmology & visual science 2008 Jul;49(7):3151-7
Investigative ophthalmology & visual science 2008 Jul;49(7):3151-7
High expression of tumor endothelial marker 7 is associated with metastasis and poor survival of patients with osteogenic sarcoma.
Fuchs B, Mahlum E, Halder C, Maran A, Yaszemski M, Bode B, Bolander M, Sarkar G
Gene 2007 Sep 15;399(2):137-43
Gene 2007 Sep 15;399(2):137-43
Chemotherapeutic stress selectively activates NF-kappa B-dependent AKT and VEGF expression in liver cancer-derived endothelial cells.
Meng F, Henson R, Patel T
American journal of physiology. Cell physiology 2007 Aug;293(2):C749-60
American journal of physiology. Cell physiology 2007 Aug;293(2):C749-60
Expression of tumor endothelial marker 7 mRNA and protein in the dorsal root ganglion neurons of the rat.
Lee HK, Kang DS, Seo IA, Choi EJ, Park HT, Park JI
Neuroscience letters 2006 Jul 10;402(1-2):71-5
Neuroscience letters 2006 Jul 10;402(1-2):71-5
Cloning, characterization and neuronal expression profiles of tumor endothelial marker 7 in the rat brain.
Lee HK, Bae HR, Park HK, Seo IA, Lee EY, Suh DJ, Park HT
Brain research. Molecular brain research 2005 May 20;136(1-2):189-98
Brain research. Molecular brain research 2005 May 20;136(1-2):189-98
Identification of a binding partner for the endothelial cell surface proteins TEM7 and TEM7R.
Nanda A, Buckhaults P, Seaman S, Agrawal N, Boutin P, Shankara S, Nacht M, Teicher B, Stampfl J, Singh S, Vogelstein B, Kinzler KW, St Croix B
Cancer research 2004 Dec 1;64(23):8507-11
Cancer research 2004 Dec 1;64(23):8507-11
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TEM7/PLXDC1 Antibody (197C193 (IM193)) [NB100-56557] - Analysis of human breast cancer lysate (35 ug per lane, RIPA buffer) at 0.03ug/ml. Band observed at ~60kDa. (Expected MW of 55.8kDa according to NP_065138.2).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TEM7/PLXDC1 Antibody (197C193 (IM193)) [NB100-56557] - Detection of TEM7 with TEM7 antibody. Human HCT-116 cell lysate probed with TEM7 antibody at 1 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: TEM7/PLXDC1 Antibody (197C193 (IM193)) [NB100-56557] - Staining of Tumor Endothelial Marker 7 in formalin-fixed, paraffin-embedded human Purkinje neurons at 2.5 ug/ml. Hematoxylin-eosin counterstain.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TEM7/PLXDC1 Antibody (197C193 (IM193)) [NB100-56557] - Formalin-fixed, paraffin-embedded human breast vessel stained with TEM7 antibody at 5 ug/ml.
