Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX121669 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX121669, RRID:AB_10721935
- Product name
- Histone H1S35ph (phospho Ser35) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Phospho-H1 Decorates the Inter-chromatid Axis and Is Evicted along with Shugoshin by SET during Mitosis.
Protein kinase A-mediated serine 35 phosphorylation dissociates histone H1.4 from mitotic chromosome.
Krishnan S, Smits AH, Vermeulen M, Reinberg D
Molecular cell 2017 Aug 17;67(4):579-593.e6
Molecular cell 2017 Aug 17;67(4):579-593.e6
Protein kinase A-mediated serine 35 phosphorylation dissociates histone H1.4 from mitotic chromosome.
Chu CS, Hsu PH, Lo PW, Scheer E, Tora L, Tsai HJ, Tsai MD, Juan LJ
The Journal of biological chemistry 2011 Oct 14;286(41):35843-51
The Journal of biological chemistry 2011 Oct 14;286(41):35843-51
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (5 μg of whole cell lysate) A: Acid-extracted histones from HeLa cell B: Acid-extracted histones from HeLa cell treated Nocodazole 12% SDS PAGE GTX121669 diluted at 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Histone H1S35ph (phospho Ser35)(GTX121669) antibody at 1:500 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Histone H1S35ph (phospho Ser35)(GTX121669) antibody at 1:500 dilution.