P06652

antibody from Boster Biological Technology

Targeting: H1-4 H1.4, H1e, H1F4, H1s-4, HIST1H1E

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

P06652

Provider

Boster Biological Technology

Product name

Anti-Phospho-Histone H1.4 (T17) HIST1H1E Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for HISTONE H1.4/HIST1H1E detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat HISTONE H1.4/HIST1H1E information: Molecular Weight: 21865 MW; Subcellular Localization: Nucleus. Chromosome. Mainly localizes in heterochromatin. Dysplays a punctuate staining pattern in the nucleus.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

DGH-8

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded mouse colon, using Phospho-Histone H14 (T17) Antibody(P06652)HIST1H1E was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HIST1H1E Antibody (P06652)overnight at 4?? Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image