LS-C20229

antibody from LSBio

Targeting: WNT4 WNT-4

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C20229

Provider

LSBio

Proper citation

LifeSpan Cat#LS-C20229, RRID:AB_2241598

Product name

WNT4 Antibody (aa23-36) LS-C20229

Antibody type

Polyclonal

Description

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Reactivity

Human, Mouse, Rat, Chicken/Avian, Porcine, Simian, Xenopus, Zebrafish

Host

Goat

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Staining (0.03 ug/ml) of a) Human Ovary and b) Human Testis (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Staining (0.03 ug/ml) of a) Human Ovary and b) Human Testis (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

WNT4 antibody (1µg/ml) staining of Jurkat (A) and negative control U937 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.