25925-1-AP

antibody from Invitrogen Antibodies

Targeting: APOL2 APOL-II

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

25925-1-AP

Provider

Invitrogen Antibodies

Product name

APOL2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MNPESSIFI EDYLKYFQDQ VSRENLLQLL TDDEAWNGFV AAAELPRDEA DELRKALNKL ASHMVMKDKN RHDKDQQHRQ WFLKEFPRLK RELEDHIRKL RALAEEVEQV HRGTTIANVV SNSVGTTSGI LTLLGLGLAP FTEGISFVLL DTGMGLGAAA AVAGITCSVV ELVNKLRARA QARNLDQSGT NVAKVMKEFV GGNTPNVLTL VDNWYQVTQG IGRNIRAIRR ARANPQLGAY APPPHVIGRI SAEGGEQVER VVEGPAQAMS RGTMIVGAAT GGILLLLDVV SLAYESKHLL EGAKSESAEE LKKRAQELEG KLNFLTKIHE MLQPGQDQ (1-337 aa encoded by BC004395)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.53 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 25925-1-AP (APOL2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A431 cells were subjected to SDS PAGE followed by western blot with 25925-1-AP (APOL2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A549 cells were subjected to SDS PAGE followed by western blot with 25925-1-AP ( APOL2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 25925-1-AP (APOL2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 25925-1-AP (APOL2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 25925-1-AP ( APOL2 Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 25925-1-AP ( APOL2 Antibody) at dilution of 1:200 (under 40x lens).