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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX119559 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX119559, RRID:AB_10617706
- Product name
- RAB20 antibody [N1C3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references A Rab20-Dependent Membrane Trafficking Pathway Controls M. tuberculosis Replication by Regulating Phagosome Spaciousness and Integrity.
Interferon-γ-inducible Rab20 regulates endosomal morphology and EGFR degradation in macrophages.
Identification of an immune-regulated phagosomal Rab cascade in macrophages.
Schnettger L, Rodgers A, Repnik U, Lai RP, Pei G, Verdoes M, Wilkinson RJ, Young DB, Gutierrez MG
Cell host & microbe 2017 May 10;21(5):619-628.e5
Cell host & microbe 2017 May 10;21(5):619-628.e5
Interferon-γ-inducible Rab20 regulates endosomal morphology and EGFR degradation in macrophages.
Pei G, Schnettger L, Bronietzki M, Repnik U, Griffiths G, Gutierrez MG
Molecular biology of the cell 2015 Sep 1;26(17):3061-70
Molecular biology of the cell 2015 Sep 1;26(17):3061-70
Identification of an immune-regulated phagosomal Rab cascade in macrophages.
Pei G, Repnik U, Griffiths G, Gutierrez MG
Journal of cell science 2014 May 1;127(Pt 9):2071-82
Journal of cell science 2014 May 1;127(Pt 9):2071-82
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: HeLa 12% SDS PAGE GTX119559 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) HeLa whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB20 antibody [N1C3] (GTX119559) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using RAB20(GTX119559) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RAB20 antibody [N1C3] detects RAB20 protein at cytoplasm on human hepatocellular carcinomas by immunohistochemical analysis. Sample: Paraffin-embedded human hepatocellular carcinomas. RAB20 antibody [N1C3] (GTX119559) diluted at 1:500.
