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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-31500 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RAB20 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, HeLa (500 µM CoCl2 treatment for 24 hr and 10 µM MG132 treatment for 2 hr).
- Concentration
- 1.12 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RAB20 using 30 µg of HeLa lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a RAB20 polyclonal antibody (Product # PA5-31500) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RAB20 was performed by separating 30 µg of untreated (–) and treated (+) HeLa whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a RAB20 Polyclonal Antibody (Product # PA5-31500) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of RAB20 in methanol-fixed HeLa cells using a RAB20 polyclonal antibody (Product # PA5-31500) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RAB20 Polyclonal Antibody detects RAB20 protein at cytoplasm on human hepatocellular carcinomas by immunohistochemical analysis. Sample: Paraffin-embedded human hepatocellular carcinomas. RAB20 Polyclonal Antibody (Product # PA5-31500) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
