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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [6]
- Other assay [1]
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- Product number
- TA500730 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500730, RRID:AB_11124238
- Product name
- Anti-BTN3A2 mouse monoclonal antibody, clone OTI1A6 (formerly 1A6)
- Antibody type
- Monoclonal
- Description
- Anti-BTN3A2 mouse monoclonal antibody, clone OTI1A6 (formerly 1A6)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- BTN3A2
- Isotype
- IgG
- Antibody clone number
- OTI1A6
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
Submitted references Butyrophilin 3A1 plays an essential role in prenyl pyrophosphate stimulation of human Vγ2Vδ2 T cells.
Wang H, Henry O, Distefano MD, Wang YC, Räikkönen J, Mönkkönen J, Tanaka Y, Morita CT
Journal of immunology (Baltimore, Md. : 1950) 2013 Aug 1;191(3):1029-42
Journal of immunology (Baltimore, Md. : 1950) 2013 Aug 1;191(3):1029-42
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTN3A2 (RC201183, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTN3A2 .
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-BTN3A2 mouse monoclonal antibody (TA500730) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTN3A2 (RC201183).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HT29 cells using anti-BTN3A2 mouse monoclonal antibody (TA500730).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-BTN3A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500730, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-BTN3A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500730, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-BTN3A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500730, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-BTN3A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500730, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-BTN3A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500730, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-BTN3A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500730, Dilution 1:50)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Vg2Vd2 T cells proliferate in response to the 20.1 but not the 1A6 mAb, although both are specific for BTN3. The 12G12 Vg2Vd2 T cell clone was cultured with control IgG1 mAb, BTN3A2(1A6) mAb, or 20.1 mAb in the presence of mitomycin Ctreated Va2 cells. The cells were pulsed with [3H]thymidine after 24 h and harvested 18 h later.
- Validation comment
- Assay
