TA500730

antibody from Invitrogen Antibodies

Targeting: BTN3A2 BTN3.2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500730

Provider

Invitrogen Antibodies

Product name

BTN3A2 Monoclonal Antibody (OTI1A6), TrueMAB™

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

OTI1A6

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTN3A2 (RC201183, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-BTN3. Positive lysates LY402082 (100 µg) and LC402082 (20 µg) can be purchased separately from OriGene.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Anti-BTN3A2 mouse monoclonal antibody (TA500730) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTN3A2 (RC201183).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of HT29 cells using anti-BTN3A2 mouse monoclonal antibody (TA500730).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-BTN3A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500730, Dilution 1:50)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-BTN3A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500730, Dilution 1:50)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-BTN3A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500730, Dilution 1:50)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-BTN3A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500730, Dilution 1:50)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-BTN3A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500730, Dilution 1:50)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-BTN3A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500730, Dilution 1:50)

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure from citation: Vg2Vd2 T cells proliferate in response to the 20.1 but not the 1A6 mAb, altho µgh both are specific for BTN3. The 12G12 Vg2Vd2 T cell clone was cultured with control IgG1 mAb, BTN3A2(1A6) mAb, or 20.1 mAb in the presence of mitomycin C–treated Va2 cells. The cells were pulsed with [3H]thymidine after 24 h and harvested 18 h later. View Citation