MA5-32817

antibody from Invitrogen Antibodies

Targeting: LOX

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-32817

Provider

Invitrogen Antibodies

Product name

LOX Recombinant Rabbit Monoclonal Antibody (JU30-23)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat, Zebrafish

Host

Rabbit

Isotype

IgG

Antibody clone number

JU30-23

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Extracellular Matrix Production by Mesenchymal Stromal Cells in Hydrogels Facilitates Cell Spreading and Is Inhibited by FGF-2.

Horton ER, Vallmajo-Martin Q, Martin I, Snedeker JG, Ehrbar M, Blache U
Advanced healthcare materials 2020 Apr;9(7):e1901669

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of LOX in Zebrafish tissue lysate using a LOX Monoclonal antibody (Product # MA5-32817) at a dilution of 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of LOX in different lysates using a Monoclonal antibody (Product #MA5-32817) at a dilution of 1:1,000. Positive control: Lane 1: Jurkat, Lane 2: Mouse brain, Lane 3: Human fetal brain.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of LOX in different lysates using a Monoclonal antibody (Product #MA5-32817) at a dilution of 1:1,000. Positive control: Lane 1: Jurkat, Lane 2: Mouse brain, Lane 3: Human fetal brain.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of LOX in Hela cells using a LOX Monoclonal antibody (Product # MA5-32817) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of LOX in HUVEC cells using a LOX Monoclonal antibody (Product # MA5-32817) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of LOX in SH-SY5Y cells using a LOX Monoclonal antibody (Product # MA5-32817) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of LOX of paraffin-embedded rat skeletal muscle tissue using a LOX Monoclonal antibody (Product #MA5-32817). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of LOX of paraffin-embedded rat skeletal muscle tissue using a LOX Monoclonal antibody (Product #MA5-32817). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of LOX of paraffin-embedded Human kidney tissue using a LOX Monoclonal antibody (Product #MA5-32817). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of LOX of paraffin-embedded Human placenta tissue using a LOX Monoclonal antibody (Product #MA5-32817). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of LOX of paraffin-embedded Mouse heart tissue using a LOX Monoclonal antibody (Product #MA5-32817). Counter stained with hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometric analysis of LOX in Hela cells using a LOX Monoclonal Antibody (Product # MA5-32817) at a dilution of 1:100, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.