10168-2-AP

antibody from Invitrogen Antibodies

Targeting: ACSM3 SA, SAH

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

10168-2-AP

Provider

Invitrogen Antibodies

Product name

ACSM3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MLARVTRKM LRHAKCFQRL AIFGSVRALH KDNRTATPQN FSNYESMKQD FKLGIPEYFN FAKDVLDQWT DKEKAGKKPS NPAFWWINRN GEEMRWSFEE LGSLSRKFAN ILSEACSLQR GDRVILILPR VPEWWLANVA CLRTGTVLIP GTTQLTQKDI LYRLQSSKAN CIITNDVLAP AVDAVASKCE NLHSKLIVSE NSREGWGNLK ELMKHASDSH TCVKTKHNEI MAIFFTSGTS GYPKMTAHTH SSFGLGLSVN GRFWLDLTPS DVMWNTSDTG WAKSAWSSVF SPWIQGACVF THHLPRFEPT SILQTLSKYP I (1-320 aa encoded by BC002790)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.17 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10168-2-AP (ACSM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of ACSM3 antibody (10168-2-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSM3 transfected HEK-293 cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10168-2-AP (ACSM3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using ACSM3 antibody 10168-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 10168-2-AP (ACSM3 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 10168-2-AP (ACSM3 Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 10168-2-AP ( ACSM3 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 10168-2-AP ( ACSM3 Antibody) at dilution of 1:50 (under 40x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HepG2 cells were stained with 0.2ug ACSM3 antibody (10168-2-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.