Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
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Validation data
Reference
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- Product number
- NB500-256 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB500-256, RRID:AB_2180012
- Product name
- Rabbit Polyclonal RCN1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Divergence between motoneurons: gene expression profiling provides a molecular characterization of functionally discrete somatic and autonomic motoneurons.
Cui D, Dougherty KJ, Machacek DW, Sawchuk M, Hochman S, Baro DJ
Physiological genomics 2006 Feb 14;24(3):276-89
Physiological genomics 2006 Feb 14;24(3):276-89
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RCN1 Antibody [NB500-256] - Whole cell lysate (5 and 15 ug) from HeLa cells, stained with RCN1 Antibody diluted at 0.2 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RCN1 Antibody [NB500-256] - Detection of human and mouse Reticulocalbin-1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Reticulocalbin-1 antibody NB500-256 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: RCN1 Antibody [NB500-256] - Detection of human Reticulocalbin-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Reticulocalbin-1 antibody NB500-256 (lot NB500-256-3) used for IP at 6 ug per reaction. Reticulocalbin-1 was also immunoprecipitated by a previous lot of this antibody (lot NB500-256-2). For blotting immunoprecipitated Reticulocalbin-1, NB500-256 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RCN1 Antibody [NB500-256] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-Reticulocalbin used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: RCN1 Antibody [NB500-256] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- RCN1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RCN1 Antibody [NB500-256] - Section of human colon carcinoma. Antibody: Affinity purified rabbit anti-RCN1 (NB500-256). Detection: DAB