PA5-58389

antibody from Invitrogen Antibodies

Targeting: SPAG6 CFAP194, CT141, FAP194, PF16, Repro-SA-1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-58389

Provider

Invitrogen Antibodies

Product name

SPAG6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: AVPLLVLCIQ EPEIALKRIA ASALSDIAKH SPELAQTVVD AGAVAHLAQM ILNPDAKLKH QILSALSQVS KHSVDLAEMV VEA Highest antigen sequence identity to the following orthologs: Mouse - 92%, Rat - 92%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.4 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Genetic interaction between central pair apparatus genes CFAP221, CFAP54, and SPEF2 in mouse models of primary ciliary dyskinesia.

McKenzie CW, Lee L
Scientific reports 2020 Jul 23;10(1):12337

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SPAG6 in control (vector only transfected HEK293T lysate) and SPAG6 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a SPAG6 Polyclonal Antibody (Product # PA5-58389).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of SPAG6 in human fallopian tube shows strong membranous positivity in cilia of glandular cells. Samples were probed using a SPAG6 Polyclonal Antibody (Product # PA5-58389).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of SPAG6 in human fallopian tube and colon tissues using SPAG6 Polyclonal Antibody (Product # PA5-58389). Corresponding SPAG6 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of SPAG6 in human colon using SPAG6 Polyclonal Antibody (Product # PA5-58389) shows low expression as expected.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Immunohistochemical analysis of ciliary markers in double homozygous sinus. Sections of WT, nm1054/nm1054;bgh/bgh , nm1054/nm1054;Cfap54 gt/gt / Cfap54 gt/gt , and bgh/bgh;Cfap54 gt/gt / Cfap54 gt/gt maxillary sinus airway epithelia stained with antibodies to ciliary marker acetylated tubulin ( a - d , a '- d '), basal body marker gamma-tubulin ( f - i , f '- i '), CPA marker SPAG6 ( k - n , k '- n '), and radial spoke marker RSPH4A ( p - s , p '- s '). The arrowhead ( a ) indicates the motile cilia, and the arrow ( f ) indicates the basal bodies. Quantification of acetylated tubulin, gamma-tubulin, SPAG6, and RSPH4A staining intensity is shown in ( e , j , o , t , respectively). nm1054;bgh: nm1054/nm1054;bgh/bgh , nm1054;Cfap54 gt/gt : nm1054/nm1054;Cfap54 gt/gt / Cfap54 gt/gt , bgh: Cfap54 gt/gt : bgh/bgh;Cfap54 gt/gt / Cfap54 gt/gt . All p values are relative to WT. For acetylated tubulin ( e ), p = 0.479 for nm1054/nm1054;Cfap54 gt/gt / Cfap54 gt/gt and 0.977 for bgh/bgh;Cfap54 gt/gt / Cfap54 gt/gt mice. For gamma tubulin ( j ), p = 0.871 for nm1054/nm1054;Cfap54 gt/gt / Cfap54 gt/gt and 0.357 for bgh/bgh;Cfap54 gt/gt / Cfap54 gt/gt mice. For SPAG6 ( o ), p = 0.772 for nm1054/nm1054;Cfap54 gt/gt / Cfap54 gt/gt and 0.253 for bgh/bgh;Cfap54 gt/gt / Cfap54 gt/gt mice. For RSPH4A ( t ), p = 0.804 for nm1054/nm1054;Cfap54 gt/gt / Cfap54 gt/gt and 0.999 for bgh/bgh;Cfap54 gt/gt / Cfap54 gt/gt mice. Statistical significance was determined by one-way ANOVA. Since only on