TA347280

antibody from OriGene

Targeting: RUNX1T1 AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2

ELISA (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

TA347280

Provider

OriGene

Product name

Rabbit Polyclonal ETO Antibody

Antibody type

Polyclonal

Description

Rabbit Polyclonal ETO Antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

RUNX1T1

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µl

Concentration

not determined

ELISA

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against human ETO. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,300.

Validation comment

ELISA

Other assay

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

The IP€™d DNA of 6 ChIP€™s was pooled and analysed with an Illumina Genome Analyzer. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Image shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.

Validation comment

Assay

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

ChIP assays were performed using SKNO-1 cells, the antibody against ETO and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 ul of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2, OGG1 and VEGF genes. Image shows the occupancy, calculated as the ratio + control/background for which the H2B gene was used.

Validation comment

Assay