H00000862-D01P

antibody from Abnova Corporation

Targeting: RUNX1T1 AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000862-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000862-D01P, RRID:AB_1579958

Product name

RUNX1T1 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human RUNX1T1 protein.

Antigen sequence

MPDRTEKHSTMPDSPVDVKTQSRLTPPTMPPPPTT
QGAPRTSSFTPTTLTNGTSHSPTALNGAPSPPNGF
SNGPSSSSSSSLANQQLPPACGARQLSKLKRFLTT
LQQFGNDISPEIGERVRTLVLGLVNSTLTIEEFHS
KLQEATNFPLRPFVIPFLKANLPLLQRELLHCARL
AKQNPAQYLAQHEQLLLDASTTSPVDSSELLLDVN
ENGKRRTPDRTKENGFDREPLHSEHPSKRPCTISP
GQRYSPNNGLSYQPNGLPHPTPPPPQHYRLDDMAI
AHHYRDSYRHPSHRDLRDRNRPMGLHGTRQEEMID
HRLTDREWAEEWKHLDHLLNCIMDMVEKTRRSLTV
LRRCQEADREELNYWIRRYSDAEDLKKGGGSSSSH
SRQQSPVNPDPVALDAHREFLHRPASGYVPEEIWK
KAEEAVNEVKRQAMTELQKAVSEAERKAHDMITTE
RAKMERTVAEAKRQAAEDALAVINQQEDSSESCWN
CGRKASETCSGCNTARYCGSFCQHKDWEKHHHICG
QTLQAQQQGDTPAVSSSVTPNSGAGSPMDTPPAAT
PRSTTPGTPSTIETTPR


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of RUNX1T1 expression in transfected 293T cell line (H00000862-T01) by RUNX1T1 MaxPab polyclonal antibody.Lane 1: RUNX1T1 transfected lysate(64.40 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between RUNX1T1 and HDAC1. HeLa cells were stained with anti-RUNX1T1 rabbit purified polyclonal 1:1200 and anti-HDAC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)