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LearnNBP2-59232
antibody from Novus Biologicals
Targeting: RUNX1T1
AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- ELISA [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- NBP2-59232 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal RUNX1T1/ETO Antibody
- Antibody type
- Polyclonal
- Description
- Whole Antiserum.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: RUNX1T1/ETO Antibody [NBP2-59232] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human ETO. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:1,300.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation: RUNX1T1/ETO Antibody [NBP2-59232] - ChIP assays were performed using SKNO-1 cells, the antibody against ETO and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 uL of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2, OGG1 and VEGF genes. Figure shows the occupancy, calculated as the ratio + control/background for which the H2B gene was used.
