Antibody data
- Antibody Data
- Antigen structure
- References [28]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [4]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF3369 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat SOX1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human SOX1 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant human (rh) SOX2 is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
NP_005977
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Generation of iPSCs carrying a common LRRK2 risk allele for in vitro modeling of idiopathic Parkinson's disease.
Rapid functional genetics of the oligodendrocyte lineage using pluripotent stem cells.
Improving Cell Survival in Injected Embryos Allows Primed Pluripotent Stem Cells to Generate Chimeric Cynomolgus Monkeys.
TFAP2C regulates transcription in human naive pluripotency by opening enhancers.
Highly efficient methods to obtain homogeneous dorsal neural progenitor cells from human and mouse embryonic stem cells and induced pluripotent stem cells.
Chronic Electrical Stimulation Promotes the Excitability and Plasticity of ESC-derived Neurons following Glutamate-induced Inhibition In vitro.
Combinatorial Smad2/3 Activities Downstream of Nodal Signaling Maintain Embryonic/Extra-Embryonic Cell Identities during Lineage Priming.
Coordinated Control of mRNA and rRNA Processing Controls Embryonic Stem Cell Pluripotency and Differentiation.
Wnt signal activation induces midbrain specification through direct binding of the beta-catenin/TCF4 complex to the EN1 promoter in human pluripotent stem cells.
A hypomorphic PIGA gene mutation causes severe defects in neuron development and susceptibility to complement-mediated toxicity in a human iPSC model.
Differentiation of oligodendrocyte progenitor cells from dissociated monolayer and feeder-free cultured pluripotent stem cells.
Direct induction of neural progenitor cells transiently passes through a partially reprogrammed state.
Dynamic behaviour of human neuroepithelial cells in the developing forebrain.
Cytosine-5 RNA Methylation Regulates Neural Stem Cell Differentiation and Motility.
Defined Culture Conditions Accelerate Small-molecule-assisted Neural Induction for the Production of Neural Progenitors from Human-induced Pluripotent Stem Cells.
Transcriptome analysis reveals determinant stages controlling human embryonic stem cell commitment to neuronal cells.
Human iPSC-Derived Cerebral Organoids Model Cellular Features of Lissencephaly and Reveal Prolonged Mitosis of Outer Radial Glia.
Modeling the Mutational and Phenotypic Landscapes of Pelizaeus-Merzbacher Disease with Human iPSC-Derived Oligodendrocytes.
Induced Pluripotent Stem Cells for Disease Modeling and Evaluation of Therapeutics for Niemann-Pick Disease Type A.
Design of a Vitronectin-Based Recombinant Protein as a Defined Substrate for Differentiation of Human Pluripotent Stem Cells into Hepatocyte-Like Cells.
Automated, high-throughput derivation, characterization and differentiation of induced pluripotent stem cells.
Lineage-specific splicing of a brain-enriched alternative exon promotes glioblastoma progression.
Derivation and long-term culture of transgene-free human induced pluripotent stem cells on synthetic substrates.
iPSC-Derived neural stem cells act via kinase inhibition to exert neuroprotective effects in spinal muscular atrophy with respiratory distress type 1.
Derivation and expansion using only small molecules of human neural progenitors for neurodegenerative disease modeling.
Transcription factor-mediated reprogramming of fibroblasts to expandable, myelinogenic oligodendrocyte progenitor cells.
Translating dosage compensation to trisomy 21.
Sonic hedgehog signaling controls thalamic progenitor identity and nuclei specification in mice.
Marrone L, Bus C, Schöndorf D, Fitzgerald JC, Kübler M, Schmid B, Reinhardt P, Reinhardt L, Deleidi M, Levin T, Meixner A, Klink B, Glatza M, Gloeckner CJ, Gasser T, Sterneckert J
PloS one 2018;13(3):e0192497
PloS one 2018;13(3):e0192497
Rapid functional genetics of the oligodendrocyte lineage using pluripotent stem cells.
Lager AM, Corradin OG, Cregg JM, Elitt MS, Shick HE, Clayton BLL, Allan KC, Olsen HE, Madhavan M, Tesar PJ
Nature communications 2018 Sep 13;9(1):3708
Nature communications 2018 Sep 13;9(1):3708
Improving Cell Survival in Injected Embryos Allows Primed Pluripotent Stem Cells to Generate Chimeric Cynomolgus Monkeys.
Kang Y, Ai Z, Duan K, Si C, Wang Y, Zheng Y, He J, Yin Y, Zhao S, Niu B, Zhu X, Liu L, Xiang L, Zhang L, Niu Y, Ji W, Li T
Cell reports 2018 Nov 27;25(9):2563-2576.e9
Cell reports 2018 Nov 27;25(9):2563-2576.e9
TFAP2C regulates transcription in human naive pluripotency by opening enhancers.
Pastor WA, Liu W, Chen D, Ho J, Kim R, Hunt TJ, Lukianchikov A, Liu X, Polo JM, Jacobsen SE, Clark AT
Nature cell biology 2018 May;20(5):553-564
Nature cell biology 2018 May;20(5):553-564
Highly efficient methods to obtain homogeneous dorsal neural progenitor cells from human and mouse embryonic stem cells and induced pluripotent stem cells.
Zhang M, Ngo J, Pirozzi F, Sun YP, Wynshaw-Boris A
Stem cell research & therapy 2018 Mar 15;9(1):67
Stem cell research & therapy 2018 Mar 15;9(1):67
Chronic Electrical Stimulation Promotes the Excitability and Plasticity of ESC-derived Neurons following Glutamate-induced Inhibition In vitro.
Latchoumane CV, Jackson L, Sendi MSE, Tehrani KF, Mortensen LJ, Stice SL, Ghovanloo M, Karumbaiah L
Scientific reports 2018 Jul 19;8(1):10957
Scientific reports 2018 Jul 19;8(1):10957
Combinatorial Smad2/3 Activities Downstream of Nodal Signaling Maintain Embryonic/Extra-Embryonic Cell Identities during Lineage Priming.
Senft AD, Costello I, King HW, Mould AW, Bikoff EK, Robertson EJ
Cell reports 2018 Aug 21;24(8):1977-1985.e7
Cell reports 2018 Aug 21;24(8):1977-1985.e7
Coordinated Control of mRNA and rRNA Processing Controls Embryonic Stem Cell Pluripotency and Differentiation.
Corsini NS, Peer AM, Moeseneder P, Roiuk M, Burkard TR, Theussl HC, Moll I, Knoblich JA
Cell stem cell 2018 Apr 5;22(4):543-558.e12
Cell stem cell 2018 Apr 5;22(4):543-558.e12
Wnt signal activation induces midbrain specification through direct binding of the beta-catenin/TCF4 complex to the EN1 promoter in human pluripotent stem cells.
Kim JY, Lee JS, Hwang HS, Lee DR, Park CY, Jung SJ, You YR, Kim DS, Kim DW
Experimental & molecular medicine 2018 Apr 13;50(4):24
Experimental & molecular medicine 2018 Apr 13;50(4):24
A hypomorphic PIGA gene mutation causes severe defects in neuron development and susceptibility to complement-mediated toxicity in a human iPSC model.
Yuan X, Li Z, Baines AC, Gavriilaki E, Ye Z, Wen Z, Braunstein EM, Biesecker LG, Cheng L, Dong X, Brodsky RA
PloS one 2017;12(4):e0174074
PloS one 2017;12(4):e0174074
Differentiation of oligodendrocyte progenitor cells from dissociated monolayer and feeder-free cultured pluripotent stem cells.
Yamashita T, Miyamoto Y, Bando Y, Ono T, Kobayashi S, Doi A, Araki T, Kato Y, Shirakawa T, Suzuki Y, Yamauchi J, Yoshida S, Sato N
PloS one 2017;12(2):e0171947
PloS one 2017;12(2):e0171947
Direct induction of neural progenitor cells transiently passes through a partially reprogrammed state.
Gao R, Xiu W, Zhang L, Zang R, Yang L, Wang C, Wang M, Wang M, Yi L, Tang Y, Gao Y, Wang H, Xi J, Liu W, Wang Y, Wen X, Yu Y, Zhang Y, Chen L, Chen J, Gao S
Biomaterials 2017 Mar;119:53-67
Biomaterials 2017 Mar;119:53-67
Dynamic behaviour of human neuroepithelial cells in the developing forebrain.
Subramanian L, Bershteyn M, Paredes MF, Kriegstein AR
Nature communications 2017 Jan 31;8:14167
Nature communications 2017 Jan 31;8:14167
Cytosine-5 RNA Methylation Regulates Neural Stem Cell Differentiation and Motility.
Flores JV, Cordero-Espinoza L, Oeztuerk-Winder F, Andersson-Rolf A, Selmi T, Blanco S, Tailor J, Dietmann S, Frye M
Stem cell reports 2017 Jan 10;8(1):112-124
Stem cell reports 2017 Jan 10;8(1):112-124
Defined Culture Conditions Accelerate Small-molecule-assisted Neural Induction for the Production of Neural Progenitors from Human-induced Pluripotent Stem Cells.
Walsh P, Truong V, Hill C, Stoflet ND, Baden J, Low WC, Keirstead SA, Dutton JR, Parr AM
Cell transplantation 2017 Dec;26(12):1890-1902
Cell transplantation 2017 Dec;26(12):1890-1902
Transcriptome analysis reveals determinant stages controlling human embryonic stem cell commitment to neuronal cells.
Li Y, Wang R, Qiao N, Peng G, Zhang K, Tang K, Han JJ, Jing N
The Journal of biological chemistry 2017 Dec 1;292(48):19590-19604
The Journal of biological chemistry 2017 Dec 1;292(48):19590-19604
Human iPSC-Derived Cerebral Organoids Model Cellular Features of Lissencephaly and Reveal Prolonged Mitosis of Outer Radial Glia.
Bershteyn M, Nowakowski TJ, Pollen AA, Di Lullo E, Nene A, Wynshaw-Boris A, Kriegstein AR
Cell stem cell 2017 Apr 6;20(4):435-449.e4
Cell stem cell 2017 Apr 6;20(4):435-449.e4
Modeling the Mutational and Phenotypic Landscapes of Pelizaeus-Merzbacher Disease with Human iPSC-Derived Oligodendrocytes.
Nevin ZS, Factor DC, Karl RT, Douvaras P, Laukka J, Windrem MS, Goldman SA, Fossati V, Hobson GM, Tesar PJ
American journal of human genetics 2017 Apr 6;100(4):617-634
American journal of human genetics 2017 Apr 6;100(4):617-634
Induced Pluripotent Stem Cells for Disease Modeling and Evaluation of Therapeutics for Niemann-Pick Disease Type A.
Long Y, Xu M, Li R, Dai S, Beers J, Chen G, Soheilian F, Baxa U, Wang M, Marugan JJ, Muro S, Li Z, Brady R, Zheng W
Stem cells translational medicine 2016 Dec;5(12):1644-1655
Stem cells translational medicine 2016 Dec;5(12):1644-1655
Design of a Vitronectin-Based Recombinant Protein as a Defined Substrate for Differentiation of Human Pluripotent Stem Cells into Hepatocyte-Like Cells.
Nagaoka M, Kobayashi M, Kawai C, Mallanna SK, Duncan SA
PloS one 2015;10(8):e0136350
PloS one 2015;10(8):e0136350
Automated, high-throughput derivation, characterization and differentiation of induced pluripotent stem cells.
Paull D, Sevilla A, Zhou H, Hahn AK, Kim H, Napolitano C, Tsankov A, Shang L, Krumholz K, Jagadeesan P, Woodard CM, Sun B, Vilboux T, Zimmer M, Forero E, Moroziewicz DN, Martinez H, Malicdan MC, Weiss KA, Vensand LB, Dusenberry CR, Polus H, Sy KT, Kahler DJ, Gahl WA, Solomon SL, Chang S, Meissner A, Eggan K, Noggle SA
Nature methods 2015 Sep;12(9):885-92
Nature methods 2015 Sep;12(9):885-92
Lineage-specific splicing of a brain-enriched alternative exon promotes glioblastoma progression.
Ferrarese R, Harsh GR 4th, Yadav AK, Bug E, Maticzka D, Reichardt W, Dombrowski SM, Miller TE, Masilamani AP, Dai F, Kim H, Hadler M, Scholtens DM, Yu IL, Beck J, Srinivasasainagendra V, Costa F, Baxan N, Pfeifer D, von Elverfeldt D, Backofen R, Weyerbrock A, Duarte CW, He X, Prinz M, Chandler JP, Vogel H, Chakravarti A, Rich JN, Carro MS, Bredel M
The Journal of clinical investigation 2014 Jul;124(7):2861-76
The Journal of clinical investigation 2014 Jul;124(7):2861-76
Derivation and long-term culture of transgene-free human induced pluripotent stem cells on synthetic substrates.
Villa-Diaz LG, Kim JK, Lahann J, Krebsbach PH
Stem cells translational medicine 2014 Dec;3(12):1410-7
Stem cells translational medicine 2014 Dec;3(12):1410-7
iPSC-Derived neural stem cells act via kinase inhibition to exert neuroprotective effects in spinal muscular atrophy with respiratory distress type 1.
Simone C, Nizzardo M, Rizzo F, Ruggieri M, Riboldi G, Salani S, Bucchia M, Bresolin N, Comi GP, Corti S
Stem cell reports 2014 Aug 12;3(2):297-311
Stem cell reports 2014 Aug 12;3(2):297-311
Derivation and expansion using only small molecules of human neural progenitors for neurodegenerative disease modeling.
Reinhardt P, Glatza M, Hemmer K, Tsytsyura Y, Thiel CS, Höing S, Moritz S, Parga JA, Wagner L, Bruder JM, Wu G, Schmid B, Röpke A, Klingauf J, Schwamborn JC, Gasser T, Schöler HR, Sterneckert J
PloS one 2013;8(3):e59252
PloS one 2013;8(3):e59252
Transcription factor-mediated reprogramming of fibroblasts to expandable, myelinogenic oligodendrocyte progenitor cells.
Najm FJ, Lager AM, Zaremba A, Wyatt K, Caprariello AV, Factor DC, Karl RT, Maeda T, Miller RH, Tesar PJ
Nature biotechnology 2013 May;31(5):426-33
Nature biotechnology 2013 May;31(5):426-33
Translating dosage compensation to trisomy 21.
Jiang J, Jing Y, Cost GJ, Chiang JC, Kolpa HJ, Cotton AM, Carone DM, Carone BR, Shivak DA, Guschin DY, Pearl JR, Rebar EJ, Byron M, Gregory PD, Brown CJ, Urnov FD, Hall LL, Lawrence JB
Nature 2013 Aug 15;500(7462):296-300
Nature 2013 Aug 15;500(7462):296-300
Sonic hedgehog signaling controls thalamic progenitor identity and nuclei specification in mice.
Vue TY, Bluske K, Alishahi A, Yang LL, Koyano-Nakagawa N, Novitch B, Nakagawa Y
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 Apr 8;29(14):4484-97
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 Apr 8;29(14):4484-97
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human SOX1 by Simple WesternTM. Simple Western lane view shows lysates of undifferentiated iBJ6 human iPS cells and iBJ6 human iPS cells differentiated into neuroprogenitor cells, loaded at 0.2 mg/mL. A specific band was detected for SOX1 at approximately 50 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human SOX1 by Western Blot. Western blot shows lysates of undifferentiated iBJ6 human iPS cells and iBJ6 human iPS cells differentiated into neuroprogenitor cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SOX1 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- SOX1 in differentiated NTera-2 Human Cell Line. SOX1 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line differentiated with retinoic acid using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green, upper panel; Catalog # NL003) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- SOX1 in Rat Cortical Stem Cells. SOX1 was detected in immersion fixed rat cortical stem cells (Catalog # NSC001) using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- SOX1 in Mouse Cortical Stem Cells. SOX1 was detected in immersion fixed mouse cortical stem cells (Catalog # NSC002) using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- SOX1 in ectoderm differentuiated BG01V Human Embryonic Stem Cells. SOX1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into neural progenitor cells using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Nestin was also detected using Mouse Anti-Mouse/Rat Nestin Monoclonal Antibody (Catalog # MAB2736) and stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009). Specific staining of SOX1 was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.