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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PA5-72201 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-PIP4K2A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 1.47 mg/ml
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PIP4K2A in HeLa cells. Samples were first fixed with 4% paraformaldehyde and permeabilized wit 0.1% Triton X-100. Cells were incubated with PIP4K2A polyclonal antibody (Product # PA5-72201) using a dilution of 1:25. Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) with a dilution of 1:400 was used as the secondary antibody. Cytoplasmic actin was detected with Alexa Fluor 555 conjugated with Phalloidin at 1:100 dilution (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PIP4K2A in paraffin-embedded human brain tissue. Samples were incubated with PIP4K2A polyclonal antibody (Product # PA5-72201) using a dilution of 1:25. A peroxidase-conjugated goat anti-rabbit IgG secondary antibody with a dilution of 1:400 was used followed by DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of PIP4K2A in HeLa cells (green) compared to an isotype control of rabbit IgG (blue). Samples were incubated with PIP4K2A polyclonal antibody (Product # PA5-72201) using a dilution of 1:25 followed by a Alexa Fluor 488 goat anti-rabbit IgG secondary antibody with a dilution of 1:400.
