21215-1-AP

antibody from Invitrogen Antibodies

Targeting: ATOH1 bHLHa14, HATH1, MATH-1, Math1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

21215-1-AP

Provider

Invitrogen Antibodies

Product name

ATOH1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MSRLLHAEE WAEVKELGDH HRQPQPHHLP QPPPPPQPPA TLQAREHPVY PPELSLLDST DPRAWLAPTL QGICTARAAQ YLLHSPELGA SEAAAPRDEV DGRGELVRRS SGGASSSKSP GPVKVREQLC KLKGGVVVDE LGCSRQRAPS SKQVNGVQKQ RRLAANARER RRMHGLNHAF DQLRNVIPSF NNDKKLSKYE TLQMAQIYIN ALSELLQTPS GGEQPPPPPA SCKSDHHHLR TAASYEGGAG NATAAGAQQA SGGSQRPTPP GSCRTRFSAP ASAGGYSVQL DALHFSTFED SALTAMMAQK NLSPSLPGSI LQPVQEENSK TSPRSHRSDG EFSPHSHYSD SDEAS (1-354 aa encoded by BC069594)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.34 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21215-1-AP (ATOH1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neuro-2a cells were subjected to SDS PAGE followed by western blot with 21215-1-AP ( ATOH1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 21215-1-AP (ATOH1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IF result of ATOH1 antibody (21215-1-AP, 1:500) with 4% PFA fixed embryonic mouse tissue/brain bu Dr. Joanna Yeung, University of British Columbia.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using (Product # 21215-1-AP) (ATOH1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).