NBP1-81580

antibody from Novus Biologicals

Targeting: RBM3 IS1-RNPL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-81580

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-81580, RRID:AB_11023848

Product name

Rabbit Polyclonal RBM3 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human RBM3 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Generation of monospecific antibodies based on affinity capture of polyclonal antibodies.

Hjelm B, Forsström B, Igel U, Johannesson H, Stadler C, Lundberg E, Ponten F, Sjöberg A, Rockberg J, Schwenk JM, Nilsson P, Johansson C, Uhlén M
Protein science : a publication of the Protein Society 2011 Nov;20(11):1824-35

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High nuclear RBM3 expression is associated with an improved prognosis in colorectal cancer.

Hjelm B, Brennan DJ, Zendehrokh N, Eberhard J, Nodin B, Gaber A, Pontén F, Johannesson H, Smaragdi K, Frantz C, Hober S, Johnson LB, Påhlman S, Jirström K, Uhlen M
Proteomics. Clinical applications 2011 Dec;5(11-12):624-35

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Nuclear expression of the RNA-binding protein RBM3 is associated with an improved clinical outcome in breast cancer.

Jögi A, Brennan DJ, Rydén L, Magnusson K, Fernö M, Stål O, Borgquist S, Uhlen M, Landberg G, Påhlman S, Pontén F, Jirström K
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2009 Dec;22(12):1564-74

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RBM3 Antibody [NBP1-81580] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RBM3 Antibody [NBP1-81580] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RBM3 Antibody [NBP1-81580] - Analysis in human cell line HeLa.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: RBM3 Antibody [NBP1-81580] - Staining of human skin shows moderate nuclear positivity in epidermal cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: RBM3 Antibody [NBP1-81580] - Staining of human kidney shows no nuclear positivity in cells in glomeruli as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: RBM3 Antibody [NBP1-81580] - Staining of human colorectal cancer shows weak nuclear positivity in tumor cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: RBM3 Antibody [NBP1-81580] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: RBM3 Antibody [NBP1-81580] - Staining of human breast cancer shows moderate nuclear positivity in tumor cells.