Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Flow cytometry [2]
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Validation data
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- Product number
- PA5-141155 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TMEM67 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Meckelin in MCF-7 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. Samples were incubated in Meckelin Polyclonal antibody (Product # PA5-141155) using a dilution of 1:500 in 5% BSA at room temperature for 2 hours followed by Goat Anti-Rabbit IgG - HRP secondary antibody at a dilution of 1:5,000 for 1 hour at room temperature.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Meckelin in paraffin-embedded human breast cancer tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with Meckelin Polyclonal antibody (Product # PA5-141155) using a dilution of 1:200 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Meckelin in paraffin-embedded human placenta tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with Meckelin Polyclonal antibody (Product # PA5-141155) using a dilution of 1:100 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Meckelin in paraffin-embedded mouse kidney tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with Meckelin Polyclonal antibody (Product # PA5-141155) using a dilution of 1:100 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Meckelin in PANC-1 cells. The cells were fixed, permeabilized and stained with Meckelin Polyclonal antibody (Product # PA5-141155) using a dilution of 1:100 (red) at room temperature for an hour followed by Alexa Fluor 488-conjugated Goat anti-Rabbit IgG secondary antibody at a dilution of 1:500 for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Meckelin in PANC-1 cells. The cells were fixed, permeabilized and stained with Meckelin Polyclonal antibody (Product # PA5-141155) using a dilution of 1:100 (red) at room temperature for an hour followed by Alexa Fluor 488-conjugated Goat anti-Rabbit IgG secondary antibody at a dilution of 1:500 for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).