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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MA5-33155 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HIST1H2BK Recombinant Rabbit Monoclonal Antibody (4G10)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 4G10
- Vial size
- 100 µL
- Concentration
- 1.5 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of HIST1H2BK using a HIST1H2BK Monoclonal antibody (Product # MA5-33155) at a concentration of 0.16 µg/mL. Positive WB detected in: Mouse brain tissue, Rat liver tissue, Rat lung tissue. All lanes: Histone H2B type 1-K antibody at 0.16µg/ml. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 14 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HIST1H2BK in paraffin embedded human testis tissue using a HIST1H2BK monoclonal antibody (Product # MA5-33155) at a dilution of 1:100. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
