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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- LS-C150044 - Provider product page
- Provider
- LSBio
- Product name
- Anti-PTF1A Antibody LS-C150044
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 500µl
- Storage
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection of Human PTF1A by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 1 ug/ml of Goat Anti-HumanPTF1A Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti Goat IgG Secondary Antibody. A specific band was detected for PTF1A at approximately 45 kD (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- PTF1A in Human Pancreatic Cancer Cells. PTF1A was detected in immersion fixed human pancreatic cancer cells using Goat Anti-Human PTF1A Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- PTF1A in Human Pancreas. PTF1A was detected in immersion fixed paraffin-embedded sections of human pancreas using Goat Anti-Human PTF1A Antigen Affinity-purified Polyclonal Antibody at 15 ug/ml overnight at 4°C. Tissue was stained using Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei.
