PA5-142010

antibody from Invitrogen Antibodies

Targeting: MLH3

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-142010

Provider

Invitrogen Antibodies

Product name

MLH3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Additional Information: This antibody is expected to recognize isoform 1 (NP_001035197.1) and isoform 2 (NP_055196.2).

Concentration

0.5 mg/mL

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of MLH3 in HeLa cells using MLH3 Polyclonal Antibody (Product # PA5-142010). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of MLH3 in HeLa cells (blue line) using MLH3 Polyclonal Antibody (Product # PA5-142010). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.