ABIN2616324

antibody from antibodies-online

Targeting: ANGPTL8 C19orf80, RIFL, TD26

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN2616324

Provider

antibodies-online

Product name

anti-Chromosome 19 Open Reading Frame 80 (C19ORF80) antibody

Antibody type

Polyclonal

Description

Caprylic acid and ammonium sulfate precipitation

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 μg

Storage

Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Handling

Avoid freeze-thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Histone H3 phospho Ser28 antibody tested by immunofluorescence. Top: HeLa cells stained with Histone H3 phospho Ser28 antibody at a 1:1,000 dilution. Middle: Same cells stained with DAPI. Bottom: Merge of both images.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Histone H3 phospho Ser28 antibody specificity tested by peptide array analysis. Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H3 phospho Ser28 antibody was applied at a dilution of 1:3,000 to Active Motif's MODified? Histone Peptide Array (Catalog No. 13001). The arrays were scanned with ArrayAnalysis Software 7 and the results plotted. Specificity data is shown for the most reactive peptides and those related to the immunogen. Recognition of the Histone H3 phospho Ser28 peptides by the antibody is inhibited by Lys27 monomethylation and blocked by Arg26 dimethylation and a citrulline at position 26. Array Data File