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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- TA300519 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA300519, RRID:AB_2170338
- Product name
- Rabbit Monoclonal Antibody against PRKAG1 (Clone Y308)
- Antibody type
- Monoclonal
- Description
- Rabbit Monoclonal Antibody against PRKAG1 (Clone Y308)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- Y308
- Vial size
- 100ul
Submitted references Chemical genetic screen identifies Gapex-5/GAPVD1 and STBD1 as novel AMPK substrates.
Ducommun S, Deak M, Zeigerer A, Göransson O, Seitz S, Collodet C, Madsen AB, Jensen TE, Viollet B, Foretz M, Gut P, Sumpton D, Sakamoto K
Cellular signalling 2019 May;57:45-57
Cellular signalling 2019 May;57:45-57
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot - AMPK gamma 1 antibody [Y308]; Anti-AMPK gamma 1 antibody [Y308] at 1/1000 dilution + Jurkat cell lysate.Predicted band size : 38 kDa.Observed band size : 38 kDa.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- AMPK gamma 1 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 10ug of Rabbit monoclonalto AMPK gamma 1and 50ul of protein G magnetic beads (lane 1). The antibody was incubated with the Protein G beads for 10min under agitation. No antibody was added to the control (lane 2). Secondary: Mouse monoclonalSecondary Antibody to Rabbit IgG light chain (HRP) . Bands: 37kDa: AMPK gamma 1.
- Validation comment
- IP
