MA5-31866

antibody from Invitrogen Antibodies

Targeting: PRKAG1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-31866

Provider

Invitrogen Antibodies

Product name

AMPK gamma-1 Monoclonal Antibody (7H3E9)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

7H3E9

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of AMPK gamma-1 in human PRKAG1 recombinant protein. Sample was incubated with AMPK gamma-1 monoclonal antibody (Product # MA5-31866) using a dilution of 1:500-1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of AMPK gamma-1 in HEK293 (1) and PRKAG1 cell lysate. Samples were incubated with AMPK gamma-1 monoclonal antibody (Product # MA5-31866) using a dilution of 1:500-1:2000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of AMPK gamma-1 in HepG2 cells (green). Sample was incubated with AMPK gamma-1 monoclonal antibody (Product # MA5-31866) using a dilution of 1:200-1:1000 followed by DRAQ5 fluorescent DNA dye (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of AMPK gamma-1 in paraffin-embedded prostate cancer tissue. Sample was incubated with AMPK gamma-1 monoclonal antibody (Product # MA5-31866) using a dilution of 1:200-1:1000 followed by DAB staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of AMPK gamma-1 in paraffin-embedded kidney tissue. Sample was incubated with AMPK gamma-1 monoclonal antibody (Product # MA5-31866) using a dilution of 1:200-1:1000 followed by DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of AMPK gamma-1 in HepG2 cells (green). Sample was incubated with AMPK gamma-1 monoclonal antibody (Product # MA5-31866) using a dilution of 1:200-1:400 followed by negative control (red).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ELISA analysis of AMPK gamma-1 in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with AMPK gamma-1 monoclonal antibody (Product # MA5-31866) using a dilution of 1:10,000.