Ab088680

antibody from Aladdin Scientific Corporation

Targeting: PRKAG1

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

Ab088680

Provider

Aladdin Scientific Corporation

Product name

Recombinant AMPK gamma 1 Antibody

Antibody type

Recombinant

Description

Rabbit anti Human AMPK gamma 1 Antibody, Recombinant (R05-2F6), could be used for WB, IF, ICC and so on.Application:WB: 1/500-1/1000ICC/IF: 1/50-1/200Protein Function:AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

AA 1-19


Antibody clone number

R05-2F6

Vial size

100μl,10μl,1ml,50μl

Concentration

0,5 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.