Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-48515 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HIST1H2BN Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Predicted to react with mouse and bovine based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.49 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HIST1H2BN in CEM cell line lysates (35 µg/lane). Lysates were probed with a HIST1H2BN Antibody (N-term) (Product # PA5-48515).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Histone H2BN was performed by loading 20 µg of Nuclear protein extracts from BEAS-2B (lane 1), and 150 ng of Purified Histones containing H2BN (lane 2) and Histone Octamer containing canonical H2B (lane 3) in sample buffer and 5 µL Spectra Multicolor Broad Range Protein Ladder (Product # 26634) onto a 15% polyacrylamide gel. Proteins were transferred to PVDF membrane (Product # 88518) at 65V for 90 min on ice. Membrane was blocked in 5% Milk in PBST for 1 hour at room temperature. Histone H2BN was detected at approximately 15 kDa using a Histone H2BN polyclonal antibody (Product # PA5-48515) at a dilution of 1:500 in 0.5% Milk in PBST overnight at 4ºC. Followed by goat anti-rabbit alkaline phosphatase secondary antibody at a dilution of 1:20,000. Chemiluminescent detection was performed using ECF substrate. Data courtesy of the laboratory of Dr. Yvonne Fondufe-Mittendorf.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HIST1H2BN Antibdy (N-term) (Product # PA5-48515) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue. Samples were probed with a HIST1H2BN Antibdy (N-term) (Product # PA5-48515) followed by peroxidase conjugation of the secondary antibody and DAB staining.
