LS-C676798

antibody from LSBio

Targeting: H2BC15 H2B/d, H2BFD, HIST1H2BN

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

LS-C676798

Provider

LSBio

Product name

HIST1H2BN Antibody LS-C676798

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate(all treated with 30mM sodium butyrate for 4h) All Lanes: HIST1H2BC antibody at 1.35µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 14 KDa Observed band size: 14 KDa

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate(all treated with 30mM sodium butyrate for 4h) All Lanes: HIST1H2BC antibody at 1.35µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 14 KDa Observed band size: 14 KDa

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot Detected samples: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h All lanes: HIST1H2BC antibody at 1:100 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 14 kDa Observed band size: 14 kDa

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunocytochemistry analysis diluted at 1:10 and staining in Hela cells(treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunocytochemistry analysis diluted at 1:10 and staining in Hela cells(treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunocytochemistry analysis diluted at 1:10 and staining in Hela cells(treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating HIST1H2BC in A549 whole cell lysate Lane 1: Rabbit control IgG instead of Acetyl-HIST1H2BC (K24) Antibody in A549 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: Acetyl-HIST1H2BC (K24) Antibody (5µg) + A549 whole cell lysate (500µg) Lane 3: A549 whole cell lysate (20µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating HIST1H2BC in A549 whole cell lysate Lane 1: Rabbit control IgG instead of Acetyl-HIST1H2BC (K24) Antibody in A549 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: Acetyl-HIST1H2BC (K24) Antibody (5µg) + A549 whole cell lysate (500µg) Lane 3: A549 whole cell lysate (20µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating HIST1H2BC in A549 whole cell lysate Lane 1: Rabbit control IgG instead of Acetyl-HIST1H2BC (K24) Antibody in A549 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: Acetyl-HIST1H2BC (K24) Antibody (5µg) + A549 whole cell lysate (500µg) Lane 3: A549 whole cell lysate (20µg)