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LearnNB100-58962
antibody from Novus Biologicals
Targeting: TMED10
p23, P24(DELTA), p24d1, p24delta1, TMP21
ELISA
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- NB100-58962 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-58962, RRID:AB_2204351
- Product name
- Goat Polyclonal Tmp21/p23 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Goat
- Antigen sequence
YLRRFFKAKKLIE- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references TMP21 is a presenilin complex component that modulates gamma-secretase but not epsilon-secretase activity.
Chen F, Hasegawa H, Schmitt-Ulms G, Kawarai T, Bohm C, Katayama T, Gu Y, Sanjo N, Glista M, Rogaeva E, Wakutani Y, Pardossi-Piquard R, Ruan X, Tandon A, Checler F, Marambaud P, Hansen K, Westaway D, St George-Hyslop P, Fraser P
Nature 2006 Apr 27;440(7088):1208-12
Nature 2006 Apr 27;440(7088):1208-12
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Tmp21/p23 Antibody [NB100-58962] - Paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary antibody at 10 ug/mL, 1 hr incubation followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Tmp21/p23 Antibody [NB100-58962] - Paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary antibody at 10 ug/mL, 1 hr incubation followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
