Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX111496 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX111496, RRID:AB_1950170
- Product name
- eEF2K antibody [N1N3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Markers of protein synthesis are increased in fetal membranes and myometrium after human labour and delivery.
Eukaryotic elongation factor 2 kinase controls proliferation and migration of vascular smooth muscle cells.
Liong S, Lappas M
Reproduction, fertility, and development 2018 Jan;30(2):313-329
Reproduction, fertility, and development 2018 Jan;30(2):313-329
Eukaryotic elongation factor 2 kinase controls proliferation and migration of vascular smooth muscle cells.
Usui T, Nijima R, Sakatsume T, Otani K, Kameshima S, Okada M, Yamawaki H
Acta physiologica (Oxford, England) 2015 Feb;213(2):472-80
Acta physiologica (Oxford, England) 2015 Feb;213(2):472-80
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE GTX111496 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A431 (GTX27909) B: H1299 C: Hela D: Hep G2 (GTX27900) 7.5% SDS PAGE GTX111496 diluted at 1:5000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with eEF2K antibody [N1N3] (GTX111496) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed A431, using eEF-2K(GTX111496) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using eEF-2K(GTX111496) antibody at 1:500 dilution.
