Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 13510-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EEF2K Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: LSENSGDEN MSDVTFDSLP SSPSSATPHS QKLDHLHWPV FSDLDNMASR DHDHLDNHRE SENSGDSGYP SEKRGELDDP EPREHGHSYS NRKYESDEDS LGSSGRVCVE KWNLLNSSRL HLPRASAVAL EVQRLNALDL EKKIGKSILG KVHLAMVRYH EGGRFCEKGE EWDQESAVFH LEHAANLGEL EAIVGLGLMY SQLPHHILAD VSLKETEENK TKGFDYLLKA AEAGDRQSMI LVARAFDSGQ NLSPDRCQDW LEALHWYNTA LEMTDCDEGG EYDGMQDEPR YMMLAREAEM LFTGGYGLEK DPQRSGDLYT QAAEAAMEAM KGRLANQYYQ KAEEAWAQME E (376-725 aa encoded by BC032665)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.35 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PC-3 cells were subjected to SDS PAGE followed by western blot with 13510-1-AP (EEF2K antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed PC-3 cells using 13510-1-AP (EEF2K antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-EEF2K (IP:13510-1-AP, 4ug; Detection:13510-1-AP 1:500) with PC-3 cells lysate 3200ug.