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- Antibody Data
- Antigen structure
- References [1]
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- Western blot [1]
- Flow cytometry [1]
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- Product number
- MAB8054 - Provider product page
- Provider
- R&D Systems
- Product name
- Human BATF Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human BATF in ELISA and Western Blot.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q16520- Isotype
- IgG
- Antibody clone number
- 687706
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Type I interferon-enhanced IL-10 expression in human CD4 T cells is regulated by STAT3, STAT2, and BATF transcription factors.
Govender U, Corre B, Bourdache Y, Pellegrini S, Michel F
Journal of leukocyte biology 2017 May;101(5):1181-1190
Journal of leukocyte biology 2017 May;101(5):1181-1190
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human BATF by Western Blot. Western blot shows lysates of KARPAS 299 human anaplastic large cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human BATF Monoclonal Antibody (Catalog # MAB8054) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for BATF at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of BATF in Raji Human Cell Line by Flow Cytometry. Raji human Burkitt's lymphoma cell line was stained with Mouse Anti-Human BATF Monoclonal Antibody (Catalog # MAB8054, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
