16379-1-AP
antibody from Invitrogen Antibodies
Targeting: ADH5
ADH-3, ADHX, FDH, GSNOR
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [7]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- 16379-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GSNOR/ADH5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MANEVIKCK AAVAWEAGKP LSIEEIEVAP PKAHEVRIKI IATAVCHTDA YTLSGADPEG CFPVILGHEG AGIVESVGEG VTKLKAGDTV IPLYIPQCGE CKFCLNPKTN LCQKIRVTQG KGLMPDGTSR FTCKGKTILH YMGTSTFSEY TVVADISVAK IDPLAPLDKV CLLGCGISTG YGAAVNTAKL EPGSVCAVFG LGGVGLAVIM GCKVAGASRI IGVDINKDKF ARAKEFGATE CINPQDFSKP IQEVLIEMTD GGVDYSFECI GNVKVMRAAL EACHKGWGVS VVVGVAASGE EIATRPFQLV TGRTWKGTAF GGWKSVESVP KLVSEYMSKK IKVDEFVTHN LSFDEINKAF ELMHSGKSIR TVVKI (1-374 aa encoded by BC014665)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse liver tissue were subjected to SDS PAGE followed by western blot with 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse heart tissue were subjected to SDS PAGE followed by western blot with 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human liver tissue were subjected to SDS PAGE followed by western blot with 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human testis tissue were subjected to SDS PAGE followed by western blot with 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas cancer using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas cancer using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human endometrial cancer using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 (under 25x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HepG2 cells were stained with 0.2ug GSNOR,ADH5 antibody (16379-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-GSNOR,ADH5 (IP:16379-1-AP, 3ug; Detection:16379-1-AP 1:800) with mouse liver tissue lysate 4000ug.