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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-59585 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POLR3E Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: FVMWKFTQSR WVVRKEVATV TKLCAEDVKD FLEHMAVVRI NKGWEFILPY DGEFIKKHPD VVQRQHMLWT GIQAKLEKVY NLVKETMPKK PDAQ Highest antigen sequence identity to the following orthologs: Mouse - 95%, Rat - 93%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of POLR3E in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a POLR3E Polyclonal Antibody (Product # PA5-59585).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of POLR3E using POLR3E Polyclonal Antibody (Product # PA5-59585) (A) shows similar pattern to an independent POLR3E Polyclonal Antibody (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of POLR3E in human cell line U-2 OS using a POLR3E Polyclonal Antibody (Product # PA5-59585) shows localization to nucleoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of POLR3E in human stomach tissue., lower shows strong nuclear positivity in glandular cells. Samples were probed using a POLR3E Polyclonal Antibody (Product # PA5-59585).
