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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00064288-M03 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00064288-M03, RRID:AB_1582083
- Product name
- ZNF323 monoclonal antibody (M03), clone 4F7
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant ZNF323.
- Antigen sequence
MEHLGDSKLQRDVSLDSKYRETCKRDSKAEKQQAH
STGERRHRCNECGKSFTKSSVLIEHQRIHTGEKPY
ECEECGKAFSRRSSLNEHRRSHTGEKPYQCKECGK
AFSASNGLTRHRRIHTGEKPYECKVCGKAFLLSSC
LVQHQRIHTGEKRYQCRECGKAFIQNTGLFQHLRV
HTGEKPYQCSQCSKLFSKRTLLRKHQKIHTGERP- Isotype
- IgG
- Antibody clone number
- 4F7
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of ZNF323 expression in transfected 293T cell line by ZNF323 monoclonal antibody (M03), clone 4F7.Lane 1: ZNF323 transfected lysate(47.3 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged ZNF323 is 0.3 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to ZNF323 on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
