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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- TA324562 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal ERAS Antibody (N-term) (F66)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal ERAS Antibody (N-term) (F66)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- ERAS
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 2.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of ERAS Antibody (N-term) (F66) (Cat. #TA324562) in HL60 cell line lysates (35ug/lane). ERAS (arrow) was detected using the purified Pab.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of SY5Y cells stained with ERAS (N-term) (F66) antibody. SY5Y cells were incubated with TA324562 ERAS (N-term) (F66) primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) . ERAS immunoreactivity is localized to the plasma membrane of SY5Y cells.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human brain tissue reacted with ERAS antibody (N-term) (F66) (Cat.#TA324562), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- IHC
