Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP11463PU-N - Provider product page
- Provider
- OriGene
- Product name
- ERAS (N-term) rabbit polyclonal antibody, Purified
- Antibody type
- Polyclonal
- Description
- ERAS (N-term) rabbit polyclonal antibody, Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- ERAS
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- lot specific
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of ERAS Antibody (N-term) in K562 cell line lysates (35 ug/lane). ERAS (arrow) was detected using the purified Pab (1:60 dilution).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of anti-ERAS Antibody (N-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human brain tissue reacted with ERAS antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- IHC