PAB3130

antibody from Abnova Corporation

Targeting: ERAS HRAS2, HRASP

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB3130

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB3130, RRID:AB_1574735

Product name

ERAS polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of ERAS.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Human ERas gene has an upstream premature polyadenylation signal that results in a truncated, noncoding transcript.

Kameda T, Thomson JA
Stem cells (Dayton, Ohio) 2005 Nov-Dec;23(10):1535-40

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Role of ERas in promoting tumour-like properties in mouse embryonic stem cells.

Takahashi K, Mitsui K, Yamanaka S
Nature 2003 May 29;423(6939):541-5

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The human c-Ha-ras2 is a processed pseudogene inactivated by numerous base substitutions.

Miyoshi J, Kagimoto M, Soeda E, Sakaki Y
Nucleic acids research 1984 Feb 24;12(4):1821-8

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of HL-60 cell lysate (35 ug/lane) with ERAS polyclonal antibody (Cat # PAB3130).

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human brain tissue reacted with ERAS polyclonal antibody (Cat # PAB3130) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)