PA5-96504

antibody from Invitrogen Antibodies

Targeting: ELAVL3 DKFZp547J036, HUC, HUCL, MGC20653, PLE21

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96504

Provider

Invitrogen Antibodies

Product name

HuC Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Immunogen sequence: QVTGVSRGVG FIRFDKRIEA EEAIKGLNGQ KPLGAAEPIT VKFANNPSQK TGQALLTHLY QSSARRYAGP LHHQTQRFRL DNLLNMAYGV KSPLSLIARF S

Concentration

0.83 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of HuC in extracts of various cell lines using HuC Polyclonal Antibody (Product # PA5-96504) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of HuC was performed in C6 cells using HuC Polyclonal Antibody (Product # PA5-96504) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of HuC was performed in C6 cells using HuC Polyclonal Antibody (Product # PA5-96504) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of HuC in paraffin-embedded rat brain using HuC Polyclonal Antibody (Product # PA5-96504) at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of HuC in paraffin-embedded mouse brain using HuC Polyclonal Antibody (Product # PA5-96504) at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry-Immunofluorescence analysis of HuC was performed in rat brain tissue using HuC Polyclonal Antibody (Product # PA5-96504) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry-Immunofluorescence analysis of HuC was performed in mouse brain tissue using HuC Polyclonal Antibody (Product # PA5-96504) at a dilution of 1:100. Blue: DAPI for nuclear staining.