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- References [3]
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- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- ABIN950340 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Alcohol Dehydrogenase 7 (Class IV), mu Or sigma Polypeptide (ADH7) (C-Term) antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
Submitted references Human stomach class IV alcohol dehydrogenase: molecular genetic analysis.
Expression and kinetic characterization of recombinant human stomach alcohol dehydrogenase. Active-site amino acid sequence explains substrate specificity compared with liver isozymes.
Alcohol dehydrogenase of class IV (sigma sigma-ADH) from human stomach. cDNA sequence and structure/function relationships.
Cheung B, Anderson JK, Holmes RS, Beacham IR
Alcoholism, clinical and experimental research 1995 Feb;19(1):185-6
Alcoholism, clinical and experimental research 1995 Feb;19(1):185-6
Expression and kinetic characterization of recombinant human stomach alcohol dehydrogenase. Active-site amino acid sequence explains substrate specificity compared with liver isozymes.
Kedishvili NY, Bosron WF, Stone CL, Hurley TD, Peggs CF, Thomasson HR, Popov KM, Carr LG, Edenberg HJ, Li TK
The Journal of biological chemistry 1995 Feb 24;270(8):3625-30
The Journal of biological chemistry 1995 Feb 24;270(8):3625-30
Alcohol dehydrogenase of class IV (sigma sigma-ADH) from human stomach. cDNA sequence and structure/function relationships.
Farrés J, Moreno A, Crosas B, Peralba JM, Allali-Hassani A, Hjelmqvist L, Jörnvall H, Parés X
European journal of biochemistry / FEBS 1994 Sep 1;224(2):549-57
European journal of biochemistry / FEBS 1994 Sep 1;224(2):549-57
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- ADH7 Antibody (C-Term) (Cat. #AP50092PU-N) western blot analysis in Jurkat,CEM,NCI-H460,K562,MDA-MB435 cell line lysates (35μg/lane).This demonstrates the ADH7 antibody detected the ADH7 protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of ADH7 Antibody (C-Term) (Cat. #AP50092PU-N) with NCI-H460 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- ADH7 Antibody (C-Term) (Cat. #AP50092PU-N) IHC analysis in formalin fixed and paraffin embedded lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ADH7 Antibody (C-Term) for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- ADH7 Antibody (C-Term) (Cat. #AP50092PU-N) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
