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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 10951-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PDHX Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MAASWRLGC DPRLLRYLVG FPGRRSVGLV KGALGWSVSR GANWRWFHST QWLRGDPIKI LMPSLSPTME EGNIVKWLKK EGEAVSAGDA LCEIETDKAV VTLDASDDGI LAKIVVEEGS KNIRLGSLIG LIVEEGEDWK HVEIPKDVGP PPPVSKPSEP RPSPEPQISI PVKKEHIPGT LRFRLSPAAR NILEKHSLDA SQGTATGPRG IFTKEDALKL VQLKQTGKIT ESRPTPAPTA TPTAPSPLQA TAGPSYPRPV IPPVSTPGQP NAVGTFTEIP ASNIRRVIAK RLTESKSTVP HAYATADCDL GAVLKVRQDL VKDDIKVSVN DFIIKAAAVT LKQMPDVNVS WDGEGPKQLP FIDISVAVAT VKGLLTPIIK DAAAKGIQEI ADSVKALSKK ARDGKLLPEE YQGGSFSISN LGMFGIDEFT AVINPPQACI LAVGRFRPVL KLTEDEEGNA KLQQRQLITV TMSSDSRVVD DELATRFLKS FKANLENPIR LA (1-501 aa encoded by BC010389)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.29 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 10951-1-AP (PDHX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10951-1-AP (PDHX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer using 10951-1-AP (PDHX antibody) at dilution of 1:100 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-PDHX (IP:10951-1-AP, 4ug; Detection:10951-1-AP 1:5000) with HEK-293 cells lysate 2000ug.
