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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- MA5-42671 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PDCD7 Recombinant Rabbit Monoclonal Antibody (8A1Q3)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Positive test controls include: TE-1, SKOV3, Mouse testis, Mouse thymus, Rat brain. The target is usually found in the following locations: Nucleus.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 8A1Q3
- Vial size
- 100 µL
- Concentration
- 0.22 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PDCD7 in various cell lines using a PDCD7 Recombinant Monoclonal antibody (Product # MA5-42671) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 10s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PDCD7 in various cell lines using a PDCD7 Recombinant Monoclonal antibody (Product # MA5-42671) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 10s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PDCD7 in human colon carcinoma using a Recombinant Monoclonal antibody (Product # MA5-42671) at a dilution of 1:100. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol..
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PDCD7 in human colon carcinoma using a Recombinant Monoclonal antibody (Product # MA5-42671) at a dilution of 1:100. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol..
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PDCD7 in human colon carcinoma using a Recombinant Monoclonal antibody (Product # MA5-42671) at a dilution of 1:100. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol..
